Purification of Interferon by Adsorption Chromatography on Controlled Pore Glass
نویسندگان
چکیده
منابع مشابه
Protein purification: adsorption chromatography on controlled pore glass with the use of chaotropic buffers.
Chromatography on controlled pore glass in combination with chaotropic buffers makes possible, in a single step, protein purifications of several hundredfold. The new emphasis is on highly selective controllable adsorption. The method is useful for the purification and concentration of proteins from large volumes of complex media and for the purification of proteins that are poorly soluble or t...
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Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2...
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Four years' experience with a method for preparing a high-purity, low-fibrinogen, heat-treated factor VIII concentrate is reported. The process, batch adsorption of a cryoprecipitate extract with controlled-pore glass granules, removes 77 percent of the cryoprecipitate fibrinogen, resulting in a final concentrate-specific activity of 0.74 IU factor VIII per mg of protein at a yield of 194 IU fa...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1976
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-33-3-517